BCM Advanced Technology Cores @BCM_IntegMicro

Check out our new papers in Pharmacological Research - Natural Products where we use phenotypic screening to discover new flavonoids toxic to bladder cancer cells. The most interesting one is found in hopps. sciencedirect.com/science/articl…

Super resolution imaging of mitochondria using PKMito Orange allows for visualization of cristae in real time.

T-Cell Interaction assay from the Nanolive. Measures interaction between cancer cells (blue) and T-cells (red) with the interaction showing in yellow.

4-color immunofluorescence of human breast tissue sections at 20X on the Yokogawa CV8000 with our new slide holder. It can image 4 slides at a time (even with the 60X water objective).

If you want to get better at image analysis and image adjustments in ImageJ/Fiji, check out this paper by previous core members. It has many useful tips on segmentation and quantitation for beginners using the software. pubmed.ncbi.nlm.nih.gov/37498127/

H&E staining (brightfield, left) does have autofluorescence in the FITC (middle) and TRITC (right) channels so be careful when immunolabeling H&E stained slides.

Stripping with TCEP for 45 minutes can remove the fluorescence from many common dyes/secondaries (not mitotracker) to allow for multiple staining cycle of the same cells/tissues.

ATP-Live dye (green) and SiR-Actin (red) on HeLa cells grown in culture. What causes the vast heterogeneity of the ATP-live signal?

Can you combine a CRISPR screen with cell painting? Ramezani et al did with their PERISCOPE technique, combining a genomie-wide CRISPR library with cell painting to see morphological effects across the genome. pubmed.ncbi.nlm.nih.gov/39870862/

A very cool article where Graham et al use the cell painting technique to follow liver cell lineages across different treatments and time. pubmed.ncbi.nlm.nih.gov/39995868/

Large lipid droplets can cause a halo-ing artifact on the Nanolive 3D cell explorer.

Cellular dynamics of mouse immortalized osteoblasts as seen on the Nanolive 3D Cell explorer. Mitochondrial, lipid, nucleus, nucleoli, and plasma membrane can all be viewed with refractive index-based holotomography.

Nuclear Pore complexes in the nuclear membrane imaged at different levels of resolution on the Leica Stellaris 8 platform.

Check out this excellent paper showing a transcriptional coactivator compound promotes resistance to oxidative stress. pubmed.ncbi.nlm.nih.gov/38969334/ They utilized high throughput confocal microscopy to tease out the protective effects of a small molecule on mitochondrial health.

Cell Painting can be used to differentiate cells in cell culture. Below are combinations of bladder cancer cell lines (5637, UMUC3, and UMUC13) and the defining characteristics based on cell paint dyes.

Label-free visualization of mitotic chromosomes using the Nanolive 3D Cell Explorer in osteoblasts.

Check out our paper in Nature Communications (@Nature ) in which we created a fast open-source program for analyzing large cell paint datasets. pubmed.ncbi.nlm.nih.gov/39580445/

Microscopy Question of the Week... Why do some cell lines have perinuclear mitochondria (left) while others have polar mitochondria (right)? Imaged on Yokogawa CV8000 following 30min addition of Mitotracker Deep Red.