Spencer LaVere Smith @Labrigger
prof at UC Santa Barbara. https://t.co/nj3RtLGy3K Neuroscience, neuroengineering, and computation. Optics, imaging, circuitry. Sharing notes at https://t.co/NhYOb5zygg slslab.org Santa Barbara, CA Joined July 2011-
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To everyone who has been shocked and depressed by the staggering 40% cut to the BRAIN Initiative: we have a voice! Outside Witness Testimony (aka written testimony) for the FY 2025 appropriations cycle is ongoing. Deadline for the House side is Friday May 3 and the Senate side…
Learn to love the shot noise - a gallery of how raw single frames may look like for two-photon imaging: gcamp6f.com/2024/04/24/why…
Learn to love the shot noise - a gallery of how raw single frames may look like for two-photon imaging: gcamp6f.com/2024/04/24/why…
I still check posts here, but I engage more over at neuromatch.social -- if you're hanging out at bluesky or threads or whatever, you might enjoy a non-corporate, fully open and free social media platform. It's not perfect, but there are fewer nazis.
The huge cut to the BRAIN Initiative at the NIH is a real and unplanned seismic event. If you want things to go differently, contact your representatives like many of us already have. braininitiative.nih.gov/news-events/bl…
@Labrigger I am super lucky to be surrounded by people who are excited by our work @Labrigger and write me a totally catchy one like this @GoardMichael
#Cellpose remains the state-of-the-art for cellular segmentation. See our new paper, in response to Ma et al, Nat Methods 2024. Paper: biorxiv.org/content/10.110… Leaderboard results ⬇️⬇️⬇️
Are you around for the OSA Optics & the Brain meeting starting tomorrow?
Postdoc position listing is now live! We're looking for a new postdoc who wants to do holographic optogenetics in the motor system. I've got a couple funded projects that are looking for owners. Apply here (or just email me): jobs.rutgers.edu/postings/225140
Awesome.
So many people joined that we had to increase capacity. If it didn’t work for you before, or you just missed this, here’s a link that you can use to join: 2p.ece.ucsb.edu/signup_user_co… x.com/Labrigger/stat…
So many people joined that we had to increase capacity. If it didn’t work for you before, or you just missed this, here’s a link that you can use to join: 2p.ece.ucsb.edu/signup_user_co… x.com/Labrigger/stat…
Here's a direct link you can use to join: 2p.ece.ucsb.edu/signup_user_co… Some invites aren't going through. If you're still waiting, try the direct link. x.com/Labrigger/stat…
Here's a direct link you can use to join: 2p.ece.ucsb.edu/signup_user_co… Some invites aren't going through. If you're still waiting, try the direct link. x.com/Labrigger/stat…
Invites have been sent. If you have any problems accessing it, let me know. x.com/Labrigger/stat…
Invites have been sent. If you have any problems accessing it, let me know. x.com/Labrigger/stat…
Christophe Leterrier @christlet
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931 Followers 888 Following NExt generation MultiphOton NeuroImaging Consortium. NSF NeuroNex hub@Labrigger @ptrrupprecht @brunopichler @voigtvision @paqio @trose_neuro I wonder have you tried this setup and compare : arxiv.org/abs/1306.5011 Lingjie Kong is now my supervisor,and I haven't compared these two setup because our laser has built-in GVD compensation.
@ptrrupprecht @voigtvision @Labrigger @paqio @trose_neuro Depends how much compensation you need. The FSPC achieves -7500fs^2, but we often need -15000fs^2 or more. The Trebino design that @Labrigger mentioned is great, but the glass for the prism (PBH71 or equivalent) can be hard to find. Simplest solution is probably chirped mirrors
@Labrigger Please add me to this nice initiative : [email protected]
@Labrigger @brunopichler @voigtvision @paqio @trose_neuro Thanks a lot, also for the blog post! For the DIY single prism solution, I wonder how difficult is it to achieve good pointing stability?
Does anybody have experience with the Thorlabs pulse compression module for 2P microscopy? Is it worth it the 10k? thorlabs.com/newgrouppage9.… Or are there other good solutions that are easy to use? @brunopichler @voigtvision @Labrigger @paqio @trose_neuro
@Labrigger @CGrienberger @hod_dana @agetliang @AdemaRibic got it, thanks! email: [email protected]
@Labrigger @jbimaknee Thanks a lot, I'll have a look!
To everyone who has been shocked and depressed by the staggering 40% cut to the BRAIN Initiative: we have a voice! Outside Witness Testimony (aka written testimony) for the FY 2025 appropriations cycle is ongoing. Deadline for the House side is Friday May 3 and the Senate side…
@ptrrupprecht ofc there is no good solution, as you know. But it would be nice to have some gut feeling about pre-filter SNR when seeing movies. Most 2p videos I see are running average on raw. 1Hz seems reasonable. in 1p all bets are off, though.
Learn to love the shot noise - a gallery of how raw single frames may look like for two-photon imaging: gcamp6f.com/2024/04/24/why…
@trose_neuro We should make a gallery as a reference for how raw data look like. Here's a single frame before and after filtering. From biorxiv.org/content/10.110…
@BlackWidow284 @IRPatNIH @NatEyeInstitute That’s a question that we’ve worked on recently, finding that the dense and high-dimensional recurrent cortical synaptic network can support the processing behind dynamic vision.
New preprint, on 'sequence filtering'. Led by @CianaDeveau, Z Zhou. We see this as a key step forw on how cortex works. All cortical areas have dense exc-exc recurrent connectivity. What do these connections do, esp in sensory ctx? Our data say: they do dynamics/time. 1/4
Packed house today at @NIH Take Your Child To Work Day. This session is about how our brains and eyes work together to allow us to see. The kids are transfixed. Lots of surprise and enthusiasm on the optical illusions. @NIMHgov @IRPatNIH @NatEyeInstitute @BlackWidow284
@CGrienberger @Labrigger @hod_dana @agetliang @AdemaRibic Hello, I would be interested too! Thank you @Labrigger
@Labrigger @hod_dana @agetliang @AdemaRibic Hi Spencer. Can I have an account as well?
@agetliang @Labrigger @AdemaRibic I’d be happy to have an account too please
@agetliang @Labrigger created a mattermost for all things imaging