Tanner Fadero @TanFad
(he/him) Light Microscopist. Tweets about microscopy 🔬, cell biology, and optics. No new posts here, but find me on bluesky: @tanner-fadero.bsky.social Redwood City, CA Joined August 2017-
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I'm not on Twitter anymore, but if you are, you should follow this account. Or come over to bsky and follow us there!
I'm not on Twitter anymore, but if you are, you should follow this account. Or come over to bsky and follow us there!
Alright, I'm calling it. I'm over to the other place (rhymes with clue guy). Find me at my new profile: tanner dash fadero at bee ess kay why dot social dot com.
We present long-term stabilization of an oblique plane microscope (OPM). By stabilizing the remote focus unit of an OPM, we can enable long-term acquisition without light-sheet drift. The feedback signal comes from a laser, no fluorescence is expended. biorxiv.org/content/10.110…
Correction from @TanFad! It's actually the The Center for Advanced Light Microscopy (CALM) @UCSF that is building the #Snouty microscope! They have a great team there with @nico_stuurman, @KariHerrington and @micaelalasser (Instagram). A great location!😀
PSA: Heat-set inserts make stronger threads in a 3D print, and look quite tidy.
Following a poke from @RunningPhoton, I've fixed a bug in my KL divergence calculation function which means our parameter-free deconvolution approach now *really* has no hand-tuned parameters at all: beryl.mrc-lmb.cam.ac.uk/rlgc_notebook/ Feedback always welcome. x.com/JamesDManton/s…
Following a poke from @RunningPhoton, I've fixed a bug in my KL divergence calculation function which means our parameter-free deconvolution approach now *really* has no hand-tuned parameters at all: beryl.mrc-lmb.cam.ac.uk/rlgc_notebook/ Feedback always welcome. x.com/JamesDManton/s…
Did you know that nuclear pore complexes can turn up outside the nuclear envelope? Studying these, @JennySachweh, Bernhard Hampoelz and others uncover that the surrounding cellular milieu determines NPC composition via the nuclear transport system itself. #teamtomo #NPC A 🧵
Where is the optical axis? Finding it can be critical to building microscopes. Here's the back reflections at ~15cm from a collimated ~1mm⌀ 532nm laser aligned into the back of Nikon 40x0.95 air objective. Radially symmetric/concentric interference rings indicate good alignment.
Gentle Rhodamines for Live-Cell Fluorescence Microscopy | ACS Central Science pubs.acs.org/doi/10.1021/ac…

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