Excited to share our work published on Cell! @CellPressNews Great thanks to @leo_jwu and Hui! cell.com/cell/fulltext/…
1/n We developed an efficient and rapid one-step blastocyst complementation platform, demonstrating that knocking out Hesx1 in mouse blastocysts can support the reconstruction of forebrain tissues by mouse or rat stem cells.
@jiahuang2022 @CellPressNews @leo_jwu Great work, Jia! How do the differences in the pace of development between rat and mouse influence synaptic patterning and connectivity in the chimeric forebrain?
2/n All intraspecies and interspecies forebrain complementation chimeras are able to survive to adulthood, with reconstructed forebrain tissue structure and function normal.
3/n We found that non-cell autonomous mechanisms determine the size, density, and developmental pace of brain tissue, while cell-autonomous mechanisms shape the overall transcriptomic features of the rat forebrain tissue in chimeras.
4/n Interspecies forebrain complementation holds promise for generating and studying wild animal brain tissues in model organisms. Optimized blastocyst complementation system hold great potential to expand research and applications in xenogeneic organ generation.
@jiahuang2022 @CellPressNews @leo_jwu Congrats Jia!