Nathan Shaner 🏳️🌈 @NathanShaner
Specializing in biological fluorescence, luminescence, and optogenetics at UCSD. All opinions here are my own. bioluminescencehub.org San Diego, CA Joined July 2012-
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CaBLAM! Check out the @NSF #NeuroNex highlight of our new calcium indicators! neuronex.org/latest bioRxiv: doi.org/10.1101/2023.0… @_theglowup @NathanShaner @div_a @UCSDHealth @CMUniversity @CarneyInstitute #bioluminescence
I'm excited to share our new project at the @focalplane_jcs webinar on Thurs! Microtutor is an interactive remote imaging education platform, integrated into the @GlobalBioImage training portal. @annaphoton, @sci_doodles & I are leading a team of collaborators including...(1/n)
What does the nervous system of a sea urchin look like? Can we study intact organ systems in calcified animals? I’m excited to share this preprint (with @FormeryLaurent and @lab_lowe) on whole-body tissue clearing in fragile invertebrates, where we tackle those questions.
Many of us use GFP all the time in lab, but have you ever wondered what fluorescent proteins do in nature? I’m delighted to share our paper (pnas.org/doi/10.1073/pn…), hot off the presses, where we uncover new roles for FPs in intertidal sea anemones. Curious? Read on: 🧵1/n
Happy 13th Birthday to our fearless leader! 🥳 #DragonKnife
The Manor Lab is seeking research assistants at all levels and postdocs to work on some exciting novel transgenic mouse models we developed. Please spread the word and have them contact me directly if interested.
@NathanShaner Nathan, sorry for late notice. Plz spread the word and apply yourself! We'll provide thorough feedback. We'll connect groups w/ funders. Low activation energy barrier for application.
@NathanShaner Nathan, sorry for late notice. Plz spread the word and apply yourself! We'll provide thorough feedback. We'll connect groups w/ funders. Low activation energy barrier for application.
We are so thankful that the @LoogerL and @NathanShaner Labs joined our annual Zhang Lab beach outing this year! 🌴 #JointLabOuting
Jin got the chance to catch up with Roger Tsien lab alumni - and their current and former trainees - at the Janelia FPs and Biological Sensors VII conference! Great to see @DedeckerLab @StPierreLab and others tagged.
Excited to share our recent paper published in @ACSSynBio on our work developing genetically encoded bioluminescent neurotransmitter sensors for glutamate! pubs.acs.org/doi/10.1021/ac… with @GElegans1 @NathanShaner @LABrechaun @_theglowup and many others who contributed.
Today in @naturemethods: our new positively tuned voltage indicator, ASAP4e, for extended electrical recordings in the brain. ASAP4e achieves greater responsivity (>200% per 100mV vs 50% for other fluorescent voltage sensors) and better photostability. nature.com/articles/s4159…
Our new CaBLAM indicator achieves much higher contrast in living cells than previously possible using bioluminescence. Congratulations to co-first authors @_theglowup and @LABrechaun Stay tuned for updates to the preprint as we do more experiments! biorxiv.org/content/10.110…
It’s World Reef day! We must sequence the coral reef communities to make a “coral-ome”, before they disappear and with them the countless potential therapeutic peptides and fluorescent proteins with potential for biological sensors. @NathanShaner and @Genomics_Guy
FIREWORMS! These marine worms glow bright green under ultra violet light. Look, but DON'T TOUCH. Their hair-like bristles contain powerful venom, making them some of the most dangerous reef residents. 📽️ Marcelo Johan Ogata bit.ly/42dtYot
About 4 minutes of action from our best new intensiometric bioluminescent calcium indicator in HeLa cells exposed to l-histamine. Sorry for no time stamp or scale bar 🥸 Seeing ΔL/L of >30-fold in some cells 👀 w/@LABrechaun, contributions from @_theglowup, biorXiv soon🤞
Calcium people - wtf is going on with these HEK cells expressing our latest bioluminescent Ca2+ sensor? Left=histamine treatment with several cells oscillating really fast; Right=luciferin in buffer, gives some big slow oscillations! Sensor is looking awesome!! @LABrechaun
I haven’t asked a question of the Twitter community for a while - what’s everyone’s favorite (i.e., best-performing) IR/NIR FP of the biliverdin-binding variety? @manorlaboratory @aaandmoore @joachimgoedhart @TalleyJLambert
Postdoc @ewaltjackson is optimizing our expression systems with the latest fluorescent proteins. Got this stunning image of an L. pictus gastrula using one of them. Thanks @NathanShaner - found some winners here! We will share on @Addgene if/when we can bit.ly/3wUkO2t
AlphaFold and RoseTTAFold have learnt some chemistry, they can distinguish between GFP-like proteins that will form a chromophore and those that do not. @DeepMind @demishassabis @UWproteindesign @langanbiotech @DavidBarondeau @LABrechaun biorxiv.org/content/10.110…
Christophe Leterrier @christlet
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62 Followers 141 FollowingGanesh Vasan, PhD @ganeshvasanc
325 Followers 668 Following Assistant Professor - University of Minnesota Dept. of Neuroscience @UMNeurosciI just spent the last hour with my mom in a pitch dark bathroom explaining the mechanism behind this glowing plant. What products like these do, beyond all other function, is enable the telling of stories. I got to bond with her over science. That's priceless to me.
A box of living inspiration just got delivered. As a flower designer, owning the works of fellow artists to enjoy in your home is so important. A constant reminder of what's possible. Dreams need references. Thank you, @light_bio for making this bundle of endless motivation.
Heading to @HHMIJanelia to run together with @ScopeShifu @caron_ajacobs this👇fantastic event! Looking forward to creating resources that will transform the dissemination and adoption of bioimaging technologies in underserved communities.
Looking forward to welcoming international delegates to @HHMIJanelia for a comprehensive and stimulating discussion on disseminating bioimaging technologies across the globe @janeliaconf #SpreadingMicroscopy
Randy Carney kicking off our series of #Convergence TED talks with the fascinating biology of extra cellular vesicles @ucdavis @ucdavisMed @ucdavisCOE
Congratulations to @CMUniversity graduate student Alexander Silvagnoli @TestCaseAlex, lead author on a new paper in Neurophotonics @SPIEtweets! 🥳🤩 doi.org/10.1117/1.NPh.… @EricPetersenPhD #bioluminescence #neuroscience
If my next paper has a picture of a weird little guy in it this is why btw
The Codex Gigas is the largest extant medieval illuminated manuscript in the world, at a length of 92 cm. The manuscript was created in the early 13th century in the Benedictine monastery of Podlažice in Bohemia, now a region in the modern-day Czech Republic. The story is that…
CaBLAM! Check out the @NSF #NeuroNex highlight of our new calcium indicators! neuronex.org/latest bioRxiv: doi.org/10.1101/2023.0… @_theglowup @NathanShaner @div_a @UCSDHealth @CMUniversity @CarneyInstitute #bioluminescence
this is fantastic!
I present to you: the white lupin @LEGO_Group! Cluster roots included
I'm excited to share our new project at the @focalplane_jcs webinar on Thurs! Microtutor is an interactive remote imaging education platform, integrated into the @GlobalBioImage training portal. @annaphoton, @sci_doodles & I are leading a team of collaborators including...(1/n)
Wordle 1,039 X/6* ⬜⬜⬜🟩⬜ ⬜🟩⬜🟩🟩 ⬜🟩⬜🟩🟩 ⬜🟩⬜🟩🟩 ⬜🟩⬜🟩🟩 ⬜🟩🟩🟩🟩one of those
I was 3rd on an upgrade list with one first class seat available showing. My bag threw me over the top and I won! Thanks @Delta 🇵🇷
"Similar shots" moments from Google Photos sometimes strike gold. Today is hey, you've been looking like depressed garbage for years! with a perfect soundtrack
So proud of and happy for youuuuu!!!!! Its been a joy to watch you grow and thrive as a scientist, community member and human ❤️🥳💪🐣🐥🦆
Beyond excited to share that I will be pursuing my Ph.D. with @Stanford Neurosciences this Fall! 🌲 Thank you to everyone who has believed in me & supported me throughout this entire process. Extremely grateful for my family, friends, mentors, & everyone I met along the way! ❤️
Welcome back to other #FossilFriday Here is the trilobite Harpes perradiatus. This comes from the Middle Devonian (Eifelian) El Otfal Formation from Morocco. The group Harpetida appeared during the Late Cambrian and went extinct by the end of the Devonian Period.
12/12 And this is how we ended up with this new palette of bright, photostable and monomeric FPs ! Plasmids are being deposited on Addgene but feel free to request them from us, especially if you're in the EU. Only thorough testing by third parties will tell how good they are.
Actin and microtubule multi color imaging with ModLoc ! @S_LevequeFort #FOM2024 Can do in depth (40um) imaging at low 7nm localization precision
Great start to #FOM2024! Check out who I ran into during coffee break @Stefan_W_Hell
Excellent introductory lectures by L. Julien, @agtebo, @claire_deo @NathanShaner