STAR Protocols @STARProtocols
STAR Protocols offers open access peer-reviewed protocols that are structured, transparent, accessible & reproducible. Lead Editor Jeremy Petravicz @JeremyNeuro cell.com/star-protocols Joined November 2019-
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Online now: Protocol for quantitative evaluation of misfolded protein degradation using Agrobacterium-mediated expression system in Nicotiana benthamiana dlvr.it/T6Dn04 #protocol #starprotocols #cellpress
Online now: Protocol to develop a moisture-desorptive passive cooling system for electronics thermal management dlvr.it/T6DPjv #protocol #starprotocols #cellpress
Online now: Chemical cross-linking to study protein self-assembly in cellulo dlvr.it/T6B6Gc #protocol #starprotocols #cellpress
Online now: Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood dlvr.it/T69lQB #protocol #starprotocols #cellpress
Online now: Protocol for evaluating mutualistic cooperative behavior in mice using a water-reward task assay dlvr.it/T67T9H #protocol #starprotocols #cellpress
Online now: Protocol for delivery of intraoperative immunotherapy to mice by surgical debulking of subcutaneous tumors dlvr.it/T65d7g #protocol #starprotocols #cellpress
Online now: A vascularized adipose organoid model using stromal vascular fraction cells from ruminant animals dlvr.it/T63rgD #protocol #starprotocols #cellpress
We @CellReports, along with @CellCellPress @NeuroCellPress and @iScience_CP, are inviting submissions on a collaborative special issue on Brain-body interactions. @STARProtocols welcomes associated protocols. Learn more: dlvr.it/T63KQT
Online now: Protocol to analyze 3D neurodegenerative vacuoles in Drosophila melanogaster dlvr.it/T63TN3 #protocol #starprotocols #cellpress
Online now: Protocol for efficient CRISPR-Cas9-mediated fluorescent tag knockin in hard-to-transfect erythroid cell lines dlvr.it/T61DZn #protocol #starprotocols #cellpress
Online now: Protocol for large DNA transgenesis in mice using the Cas9+Bxb1 toolbox dlvr.it/T60slc #protocol #starprotocols #cellpress
Online now: Protocol for evaluating the effect of potential on electrochemical reactions via the grand canonical fixed-potential technique dlvr.it/T5ycfz #protocol #starprotocols #cellpress
Online now: Simultaneous quantitative respirometry and fluorometric assays in dissected hippocampal tissue from mice dlvr.it/T5yFKn #protocol #starprotocols #cellpress
Want to study the impact of hypoxia on the tumor microenvironment? Read this new protocol by @subhamoy_d and @RoswellPark on how to study this using photoacoustic imaging sciencedirect.com/science/articl…
In collaboration with Dr. Mukund Seshadri lab @RoswellPark we report how to take advanatage of photoacoustic imaging to study the impact of hypoxia on tumor microenvironment. Congrats to co-first authors Tao Dai and Laurie Rich.
In collaboration with Dr. Mukund Seshadri lab @RoswellPark we report how to take advanatage of photoacoustic imaging to study the impact of hypoxia on tumor microenvironment. Congrats to co-first authors Tao Dai and Laurie Rich.
Congrats to Elena Navarro-Guerrero and Roberta Baronio from the Ebner group @CmdOxford for their new paper out on an optimised protocol for CRISPR knockout of human iPSC-derived macrophages in @STARProtocols pubmed.ncbi.nlm.nih.gov/38401123/
Excited about imaging transcriptomics? Read this recently published protocol by @DanielArturAM @otta_dps @matteofrigo @AlessioGiacome4 here: sciencedirect.com/science/articl…
Online in @STARProtocols: #CRISPR #Cas12a #ribonucleoprotein-mediated gene editing in the #plant pathogenic #fungus #Magnaportheoryzae, from Dr. David E. Cook @1DEcook @KSUPlantDr: hubs.li/Q016X__20
Online now: Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay dlvr.it/T5vzGC #protocol #starprotocols #cellpress
Online now: Protocol for automated N-glycan sequencing using mass spectrometry and computer-assisted intelligent fragmentation dlvr.it/T5vcn9 #protocol #starprotocols #cellpress
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54 Followers 22 FollowingInterested in publishing your #biophysics research in @iScience_CP? Learn more by booking a meeting with senior scientific editor @RituChakra hubs.li/Q02jj_9p0 @BiophysicalSoc
Looking for a chance to flex your creative muscles? Immunity is turning 30, and we're are hosting an open call for cover submissions for our special anniversary issue! The winning design will be featured on the cover of our April 2024 anniversary issue! More info here:…
Excited to announce our latest publication in @STARProtocols! My talented undergrad trainees (April and Gustave) have put together a detailed protocol for making gene-specific split-GAL4 lines by crosses. 🧬 Read more: [sciencedirect.com/science/articl…] #ProudMentor #Drosophila #Genetics
New paper! @STARProtocols actually let me write a big ol’ protocol: live cell imaging to quantify Candida albicans escape from macrophages. star-protocols.cell.com/protocols/3144 I’m talking notes, video demonstrations, troubleshooting, sample data, MATHS! @TravenLab @CentreImpactAMR
Online now: Antigen density quantification of cell-surface immunotherapy targets by flow cytometry: Multi-antigen assay of neuroblastoma bone marrow metastasis dlvr.it/Sz2ZvX #protocol #starprotocols #cellpress
Super happy to share our methods with the science world 🌎🤩 @LydiaBeckerIII @BIG_research @wtccmr @UProteoglycans @BSMB1 @douglaspdyer
New in STAR Protocols: Chronic cranial window implantation for high-resolution intravital imaging of the endothelial glycocalyx in mouse cortex 🧠by @LydiaBeckerIII @wtccmr @AnnaGrayNeuro Read it here: star-protocols.cell.com/protocols/3122
Are you planning to perform CRISPR/Cas9 to delete TEs in Drosophila? 🧬✂️🧬Check out our (@GonzalezLab_BCN) protocol in @STARProtocols 👇 star-protocols.cell.com/protocols/2924
Excited to share our latest research on developing functional assays to classify BRCA2 variants of uncertain significance (VUS)! 🧬 Published in @PLOSGenetics, @CellRepMethods, and @STARProtocols. Huge thanks to the Sharan lab at @NCIResearchCtr and @FredNatLab! 🙌
The STAR Protocol corresponding to our recent Cell Reports article is now available! Featuring methods to study preterm birth susceptibility systemically and at the cell level @divanovic_lab @CincyImmunology @CincyMSTP @STARProtocols star-protocols.cell.com/protocols/3050
Congrats to Jennifer Wayland, Emma Stemen, and Jessica Doll on their STAR Protocol associated with our @CellReports publication on the role of BAFF in preterm birth. @JL_Wayland @StemenEmma @CincyImmunology @CincyMSTP @CincyChildrens @STARProtocols star-protocols.cell.com/protocols/3050
A technical breakthrough in our ability to preserver RNA integrity to profile key cells of the brain by @Larallbb in @STARProtocols @salkinstitute - might be of interest to some folks over at @10xGenomics @LGMartelotto @AllenInstitute
Do you want to work with EVs and DNA-seq in #Leishmania or other protozoan parasites? This could be useful: Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites - ScienceDirect sciencedirect.com/science/articl…
Thrilled to see our protocol on platelet releasate isolation published in @STARProtocols @maguirepatr @hayleymacleod7 @Shane_Comer1 @fniainle @BarryKevane @SarahKCullivan @szklannap sciencedirect.com/science/articl…
Lumen researchers present a protocol for harnessing the natural transformability of spirulina to generate strains that express heterologous proteins. Read the full article below, star-protocols.cell.com/protocols/2468… #spirulina #proteins #biotech #biotechnology #dna #seattle #wa #genotyping
This is a detailed protocol describing the CSF collection and infusion done in our recent study -“Young CSF restores oligodendrogenesis and memory in aged mice via Fgf17” nature.com/articles/s4158…. well done @AchintKaur27 and thank you @wysscoray for all your support!!
Have you ever wanted to study the cerebrospinal fluid (CSF) in rodents? Look no further, excited to share our protocol for the collection and infusion of CSF in mice 🐭
In ovo electroporation delivery of #Hyaluronan-modifying chemicals (resin beads) or enzymes (plasmids) to control tissue morphogenesis @BSanketi @KurpiosLab @STARProtocols 2023 star-protocols.cell.com/protocols/2564 Guess if this can be adapted to generate a spatially-defined #AorticAneurysm?
Want to do brain organotypic cultures for studying metastasis but might be reluctant to try by yourself? We want to encourage cancer researchers to try this great (but not perfect) model with this @STARProtocols Have a look! @CNIOStopCancer sciencedirect.com/science/articl…
2/5 We recently published a novel robotic system called #HUMITIPAA at @iScience_CP (cell.com/iscience/fullt…), had it highlighted by @Nature (nature.com/articles/d4158…), and reported its step-by-step operation at @STARProtocols (star-protocols.cell.com/protocols/2191).
Need to analyze enzymatic hydrolysis of less than 1 ng of cellulose? Check out our step-by-step protocol in @STARProtocols.
Check out this #protocol by Dr. Shigeru Deguchi @shigerudeguchi @JAMSTEC_PR @INTL_JAMSTEC using surface pitting observation technology for analyzing enzymatic hydrolysis of #cellulose: star-protocols.cell.com/protocols/2394 👉Method videos included! Research in @iScience_CP
For #FluorescenceFriday we are excited to share our first publication! We outline a pulse chase protocol to monitor distinct mitochondrial populations over time to investigate turnover in neurons. Congratulations to first author @jordanrosez! doi.org/10.1016/j.xpro…