Sudha Mishra @SudhsM7
PhD scholar at GPCR Biology Lab, BSBE Department, IIT Kanpur Joined July 2019-
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🚀 New from our lab! A genetically encoded nanobody sensor (Ib32) reveals that different GPCRs sculpt β-arrestin into distinct conformations, offering receptor-specific fingerprints of signaling. pnas.org/doi/10.1073/pn…
It was such an incredible learning experience with this phenomenal team @ParishmitaSarm2 @SudhsM7 @NashrahZaidi 🙌 And to my guide- the incredible, truly inspiring, one and only @arshukla 🌟 forever grateful for your mentorship & support! 🚀✨
@IITKanpur @LazarLab @IOCBPrague Many thanks to @IndiaDST for supporting this work through a joint Indo-Czech project! We are also immensely thankful to @India_Alliance @ANRFIndia @DBTIndia @ICMRDELHI @EMBO_YIP @CSIR_IND for supporting the research program in our laboratory over the years!
Many congratulations to all involved including amazing students and fellows from our laboratory @IITKanpur and from the laboratory of our collaborator @LazarLab @IOCBPrague !
Delighted to share our latest study on a nanobody-based sensor of GPCR activation and signaling! A genetically encoded nanobody sensor reveals conformational diversity in β-arrestins orchestrated by distinct seven transmembrane receptors | PNAS pnas.org/doi/10.1073/pn…
The goal of a PhD is not to learn some facts or read a few papers or learn a bunch of techniques. The goal of a PhD is to learn independence, problem solving, how to finish things you start, resilience, & gain the ability to adapt & think creatively. Learning these things is hard
Potato evolved from tomato 9 million years ago. cell.com/cell/fulltext/… @NHM_London Sandra Knapp @UBC Loren H. Rieseberg @SCUCN Jianquan Liu #CAAS Sanwen Huang @CellCellPress
7/n In order to understand the fascinating pharmacology and biology of these incredibly versatile receptors, we reconstituted a broad set of agonist-receptor-G-protein ternary complexes in various combinations, and determined a total of seventeen cryo-EM structures😎.
3/n The species-specific pharmacology is not limited to synthetic ligands but also extends to the natural C3aR agonists! For example, TLQP21, a VGF-derived, non-C3a agonist of C3aR, also shows a dramatic preference for the mouse receptor, especially in the beta-arrestin assay!
2/n We also observe that JR14a and SB290157, the so-called small molecule antagonists of C3aR, are actually agonists, which aligns with recent reports from other labs! Surprisingly, we discover that unlike JR14a, SB290157 is strongly selective for the human C3aR over mouse C3aR!
Excited to present our preprint on the structure-function relationship of anaphylatoxin complement receptors. A casual number of 19 structures deciphering ligand recognition mechanisms, G protein/arrestin and species specific signaling. Check it out! biorxiv.org/content/biorxi…
Excited to share our latest study, now available online. Molecular fingerprints of a convergent mechanism orchestrating diverse ligand recognition and species-specific pharmacology at the complement anaphylatoxin receptors biorxiv.org/content/10.110…
Delighted to share our latest study on the complement anaphylatoxin receptors 😎!!! Molecular fingerprints of a convergent mechanism orchestrating diverse ligand recognition and species-specific pharmacology at the complement anaphylatoxin receptors biorxiv.org/content/10.110…
Excited to share our latest cryo-EM structure of CXCR3 in complex with small-molecule agonist. Our findings reveal that these agonist induces transducer bias in CXCR3 and, interestingly, exhibits dual agonistic properties at the CXCR7. nature.com/articles/s4146…
Delighted to share our latest study on chemokine receptors, now published in @NatureComms !!! Structural visualization of small molecule recognition by CXCR3 uncovers dual-agonism in the CXCR3-CXCR7 system | Nature Communications nature.com/articles/s4146…
Delighted to share our latest work on the structural visualization of small molecule recognition by CXCR3 and uncovering of dual-agonism in the CXCR3-CXCR7 system published in Nature Communications!! nature.com/articles/s4146…
#authorinterview @SudhsM7 & @ANNUDALAL7 from lab of @arshukla @BSBEIITK1 @mfcem_iitk @IITKanpur talks about their work on "Decoding CXCR2: Unravelling Chemokine Promiscuity for Targeted Therapeutics" published in Mol Cell. biopatrika.com/academia/resea… via @biopatrika
The Paradox of Knowledge
Excited to share our latest findings on CXCR2 chemokine receptor promiscuity, revealing that a conserved motif in both the receptor and ligand governs the selective binding of multiple ligands to a single receptor. Congratulations to everyone involved. cell.com/molecular-cell…
Very excited to share our latest work on the molecular basis of promiscuous chemokine binding and structural mimicry at the C-X-C chemokine receptor, CXCR2: Molecular Cell!!! cell.com/molecular-cell…
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