Dr. rer. nat. Valentin Bruttel @VBruttel
currently not active on twitter/X father, scientist, bioengineer, immunologist. CSO Toleris Biotherapeutics. private account. supporter https://t.co/BSBx7WXTKB Germany Joined October 2019-
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“It is ludicrous to interpret two mutations as proof of distinct spillovers. This hypothesis of two early spillovers is demolished by a very recent paper which concluded All of them may have evolved from one common ancestor” — Simon Wain-Hobson biosafetynow.substack.com/p/why-words-ma…
Also, the BsaXI debate here is actually more relevant to the process of RFLP validation, which are sites which are introduced during the engineering process of a specific protein change that are intended to be reamplified and digested to track the changes of the engineering…
Much appreciated and please challenge that Feb 2019 redaction @garyruskin Please expand your UNC search terms to include HKU3 and 279, it's what he called SARS-CoV-2 in 2018 And anything regarding patent US9884895B2 jimhaslam.substack.com/p/sars2-was-pa…
🧵THREAD🧵 Do you remember how bad the media’s “Covid lab leak” - the hypothesis that the virus came from a lab - coverage was? I thought I did. But it was a more dramatic example of uniform media malpractice than even I remembered. So I revisited it. Buckle in, it’s long. ⤵️
Ah yes, the "why engineer a non-canonical furin cleavage site?" argument. But @slatestarcodex is actually wrong here on several counts: First, the SARS2 PRRAR didn't mutate "towards a more normal form". The canonical FCS is RxRR, and while in Delta the P preceding the FCS mutated…
You say, “but how about the methods that don’t leave the sites in!” x.com/daoyu15/status… Unfortunately, that also require an absence of interleaving between the BsaI and BsmBI sites and that you don’t have internal sites that are too close to each other. Both drive…
In fact, the first time the Baric team didn’t use BglI, they chose to keep all their type IIS sites in. The number if fragments is again 6. ncbi.nlm.nih.gov/pmc/articles/P… now they were also purchasing BsmBI in DEFUSE, so just swap the SapI with BsmBI (which the WIV, lacking the Baric…
gab.com/Flavinkins/pos… gab.com/Flavinkins/pos… During the construction of full-length genomes by DEFUSE, the actual methodology is “consensus sequence construction”—e.g. they gather multiple sequences, usually a pool of reads, and then during the construction process, reads…
very beautiful poem! so important to remember all those who died and all the suffering that this pandemic caused. thanks for sharing this Mona @MonaRahalkar!
very beautiful poem! so important to remember all those who died and all the suffering that this pandemic caused. thanks for sharing this Mona @MonaRahalkar! https://t.co/8VjICs0MR1
@VBruttel @gadboit @mbw61567742 @ban_epp_gofroc There's a saying that is oft repeated. "You never try to reinvent the wheel" Finding a naturally occurring syn mut would be the best choice from a bioengineering perspective.
People ask: What are the chances SARS-CoV-2 emerged near a lab specializing in SARS-like viruses? A more precise question might be: What are the chances SARS-CoV-2 emerged near the lab looking for SARS 2.0?
People ask: What are the chances SARS-CoV-2 emerged near a lab specializing in SARS-like viruses? A more precise question might be: What are the chances SARS-CoV-2 emerged near the lab looking for SARS 2.0?
@VBruttel @ydeigin @R_H_Ebright Agree. The time for politeness and equivocation is over. Covid came out of a lab and anyone with a functioning brain knows it.
Really excellent work by @ydeigin pulling together all the sources.
Excited to announce the publication of "Case-Control Study of Individuals With Small Fiber Neuropathy After COVID-19" neurology.org/doi/10.1212/NX…
Newly discovered emails... Ecohealth's Peter Daszak: "we have 15,000 samples in freezers in Wuhan" ecohealthalliance.org/wp-content/upl…
@hholdenthorp tosses @PeterDaszak under the bus, saying he was critical of how he handled the disclosure of DEFUSE in 2021 and remarking “Dr. Daszak may have had other ideas other than what was in the proposal.”
@hholdenthorp tosses @PeterDaszak under the bus, saying he was critical of how he handled the disclosure of DEFUSE in 2021 and remarking “Dr. Daszak may have had other ideas other than what was in the proposal.” https://t.co/iSMSxhsS9d
@JamieMetzl @alisonannyoung It's happening. People are jumping ship fast.
"The so-called Proximal Origins paper... sits among the most consequential failures of scientific integrity that I have seen in more than 30 years working on science and technology policy" 🔥
"The so-called Proximal Origins paper... sits among the most consequential failures of scientific integrity that I have seen in more than 30 years working on science and technology policy" 🔥
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Charles Rixey, MA MBA.. @CharlesRixey
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26K Followers 1K Following Drug developer of Alzheimer’s/CNS gene therapies https://t.co/HPgKP0qGJv | Vitalist & longevity maximalist | DRASTIC cofounder https://t.co/jpRBlDRMg1 |🇺🇦🇨🇦
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44K Followers 1K Following Journalist; Former Investigator U.S. Senate; Former Safra Ethics Center, Harvard. https://t.co/aD6NkYzjqM
@mbalter — investig.. @mbalter
12K Followers 8K Following Journalist/journo prof. Former Paris corresp/Science. Contributor @Truthdig. See also: https://t.co/1EBthERMGS and https://t.co/Wq88zcBEj5.
Ben @USMortality
63K Followers 1K Following Software Engineer • COVID-19 Research, Bioinformatics & Statistics • Articles: https://t.co/NZl9auP70m
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4K Followers 1K Following Dissecting dubious claims around the pandemic origin, mainly from the pro-zoonosis crew.
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128K Followers 3K Following Biologist | Author of Red Queen, Genome, Rational Optimist, and Viral: The Search for the #OriginOfCovid: https://t.co/wHGfoouaqx
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2K Followers 719 Following Curious about what's in Wuchang https://t.co/Qv7nUEJweE
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63K Followers 14K Following https://t.co/M3eSpDEAUu Founder • Futurist • #Superconvergence #HackingDarwin #EternalSonata author • @AtlanticCouncil • Fmr NSC, State, UN, @WHO adv committee
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101K Followers 27 Following Quotes by Friedrich Nietzsche | Philosopher | Writer | Poet ✍️ | @reachmastery | Follow on IG 👉👉 https://t.co/yJ0J9yiSox
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Cure ME/CFS (and Long.. @CureCFS
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2K Followers 1K Following“It is ludicrous to interpret two mutations as proof of distinct spillovers. This hypothesis of two early spillovers is demolished by a very recent paper which concluded All of them may have evolved from one common ancestor” — Simon Wain-Hobson biosafetynow.substack.com/p/why-words-ma…
Also, the BsaXI debate here is actually more relevant to the process of RFLP validation, which are sites which are introduced during the engineering process of a specific protein change that are intended to be reamplified and digested to track the changes of the engineering…
Much appreciated and please challenge that Feb 2019 redaction @garyruskin Please expand your UNC search terms to include HKU3 and 279, it's what he called SARS-CoV-2 in 2018 And anything regarding patent US9884895B2 jimhaslam.substack.com/p/sars2-was-pa…
greatest tweet ever x.com/VBruttel/statu…
@Rossana38510044 @WashburneAlex You could also argue that it would look very weird if they published the first ever reverse genetics system made by intoducing zero instead of 5-8 synonymous mutations. But they should have noticed, could have discussed this... Or we got it wrong, but how? Comments @Baric_Lab?
🧵THREAD🧵 Do you remember how bad the media’s “Covid lab leak” - the hypothesis that the virus came from a lab - coverage was? I thought I did. But it was a more dramatic example of uniform media malpractice than even I remembered. So I revisited it. Buckle in, it’s long. ⤵️
@VBruttel @scotub The Dutch faction of the Cult of the Zoonati apparently. h/t @Rebecca21951651
@masfique @TheMatrixStorm @DrHermiz Concur. To publish a paper one knows to be misleading demonstrates lack of ethics.
@TheMatrixStorm @DrHermiz @DrHermiz I wouldn't waste a second on his behalf unless there is a retraction of the proximal paper.
@masfique My views are based, in part, on information I cannot make public.
Ah yes, the "why engineer a non-canonical furin cleavage site?" argument. But @slatestarcodex is actually wrong here on several counts: First, the SARS2 PRRAR didn't mutate "towards a more normal form". The canonical FCS is RxRR, and while in Delta the P preceding the FCS mutated…
You say, “but how about the methods that don’t leave the sites in!” x.com/daoyu15/status… Unfortunately, that also require an absence of interleaving between the BsaI and BsmBI sites and that you don’t have internal sites that are too close to each other. Both drive…
Keep in mind that the other MERS-CoV, PgCoV, etc. in publications known, are actually all not mentioned as “consensus” genomes. arxiv.org/abs/2104.01533 Actual known consensus’s genomes, found leaked out of the WIV, were identified with several distinct cloning strategies (one…
Only BsaI and BsmBI came with detailed kit compositions and instructions that can be closely followed and guranteed to work on the get-go. You don’t want your precious synthesized cDNA to not cut and ruin your assembly if you have to then identify and dial in the digestion…
In fact, the first time the Baric team didn’t use BglI, they chose to keep all their type IIS sites in. The number if fragments is again 6. ncbi.nlm.nih.gov/pmc/articles/P… now they were also purchasing BsmBI in DEFUSE, so just swap the SapI with BsmBI (which the WIV, lacking the Baric…
Also, if they “don’t leave the sites in”, then they would not have said that they will “link by unique restriction endonuclease sites that do not disturb the coding sequence” because cutting the sites out mean there won’t be a concern to not disturb the coding sequence. You can…
gab.com/Flavinkins/pos… gab.com/Flavinkins/pos… During the construction of full-length genomes by DEFUSE, the actual methodology is “consensus sequence construction”—e.g. they gather multiple sequences, usually a pool of reads, and then during the construction process, reads…
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