Eduardo 'Yoshi' Hirata @edyoshikun
🇲🇽🇯🇵 R&D Engineer at @czbiohub || Ph.D || views are my own || (he/him) Joined January 2019-
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Our first preprint as a lab is out! This one is short and sweet: We thought there had to be a faster/higher throughput way of making transgenic fish, so we set out to test if lipofection works, and it does!
Our first preprint as a lab is out! This one is short and sweet: We thought there had to be a faster/higher throughput way of making transgenic fish, so we set out to test if lipofection works, and it does! https://t.co/Uxj6O1Pvu8
#AIRR microscopy has arrived! Use any immersion objective (including air!) to look into any sample refractive index! Built here with air, water and oil objectives, and for a sample RI in the range 1.33-1.51 (zoom lens). Could this be the fastest/most versatile light-sheet ever?
Introducing Mantis, a two-in-one microscope designed at #CZBiohubSF that enables rapid high-dimensional imaging of molecular dynamics in the context of cell morphology. 🧵1/7 Preprint: ⬇️ biorxiv.org/content/10.110…
Lost in the StayGold monomeric variants? Here's a thread to try to sort things out 🧵 In 2022, the Katayama/Miyawaki labs published StayGold, a bright and stable fluorescent protein (FP) from jellyfish C. Uchidae: nature.com/articles/s4158… @NatureBiotech
a Portrait of Tenochtitlan, my 3D reconstruction of the capital of the Aztec Empire is released! I've been looking forward to this for a long time, and I am really curious what all of you think. Take a look: tenochtitlan.thomaskole.nl #tenochtitlan
As a TA in the DL@MBL 2023 course, last two weeks at @MBLScience were fantastic! Amazing interactions with participants, TAs and the faculty, tasty #KeylimePie 🥧, and at the end working with @Anks_8D, @dz_gong and @edyoshikun on #virtualstaining and #μSplit. 🎉🔬🧑💻
DL@MBL - class of 2023. An amazing group of students and TAs, speakers, and teaching staff! Thank you all for being awesome and putting so much of yourself into this!!! #DL @AI4LifeTeam @MBLScience Next: project week, i.e. working on data and problems student brought along! 🎉
I am sharing my team's most active open-source projects in computational imaging and deep learning for bioimaging. We welcome users and contributors! This nice poster is courtesy of @edyoshikun and @ZW_Liu, presented at the recent @czbiohub confab.
We created a fully-featured @napari_imaging plugin for @metaai's incredible Segment Anything Model (SAM). It supports automatic instance segmentation and human feedback from points and bounding boxes. Try it! github.com/JoOkuma/napari… #segmentanything
Congrats @edyoshikun best poster prize at #MAF2022 and all the other students, it was great sessions!
Students from both sides of the Atlantic discussing microscopy at MAF Göteborg #MAF2022
Poster presentations with @edyoshikun at MAF Göteborg #MAF2022
SaraLab reunion for @edyoshikun PhD defense today. I'm so proud of you Dr Hirata!
Amazing creatures at the 'Deep Sea's exhibit @MontereyAq! Although some ctenophores are bioluminescent, the 'rainbow' is a diffractive effect from their beating cilia 🌈
Insanely bright and photostable GFP ⚡Live-3D imaging here we go nature.com/articles/s4158…
Maurice U. @maurice_um
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Nancy Pelosi Stock Tr.. @PelosiiTrackerr
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Tara EB, Ph.D. @TaraEssockBurns
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Stefania Marcotti @lankylaste
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YangJoon Kim @yangjoonkim
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Kristina Ulicna, PhD .. @KristinaUlicna
14K Followers 12K Following Research Scientist 👩💻 @Valence_AI 💻🧪, powered by @RecursionPharma 🔬🧬 | Visiting @Mila_Quebec in Montréal 🇨🇦 | 👩🔬 Into single cells 🧫 via #python 🐍
AVR Optics @AVR_Optics
18 Followers 101 Following AVR Optics is a US-based, internationally known company in the field of fluorescence microscopy and optics. We're about People and Science- in that order.
Hongquan Li @hongquan_li
485 Followers 384 Following
Martin L. O'Shield @WindyCitySDR
2K Followers 3K Following IBM Global Entrepreneur, Intel Embedded Systems Designer, Facebook Telecom Infra Project & member of France’s Open Air Interface Alliance https://t.co/JWaOTRzujy
Suzanne Dolly @SuzanneDol18718
141 Followers 2K Following
Adrian Jacobo @Adrian_Jacobo
2K Followers 2K Following Group Leader @czbiohub. PhD in Physics turned into a quantitative biologist/zebrafish aficionado. Husband and father.
Xiang Zhao @hoover_zhao
139 Followers 361 Following Focusing on zebrafish development in Royer’s group @CZBiohub. Postdoc from @Suguo_brainsci @UCSF. PhD from Neuroscience Center @helsinkiuni
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Keir Balla @keirballa
374 Followers 301 Following Group Leader @czbiohub in San Francisco | Foraging for viruses of zebrafish to illuminate infection biology and evolution
Bryant @BryantChhun
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Shruthi VijayKumar @Shruthi94Vijay
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Talon Chandler @TalonChandler
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Berkeley AI Research @berkeley_ai
149K Followers 190 Following We're graduate students, postdocs, faculty and scientists at the cutting edge of artificial intelligence research.
Anthropic @AnthropicAI
262K Followers 26 Following We're an AI safety and research company that builds reliable, interpretable, and steerable AI systems. Talk to our AI assistant Claude at https://t.co/aRbQ97uk4d.
Austin E. Y. T. Lefeb.. @Austin_Lefebvre
726 Followers 519 Following Dr. Leafbeaver, DS @calico. You can't spell imaging without "im aging" and boy am I. Organelle enthusiast. Creator of Nellie and Mitometer.
Stephan Preibisch @preibischs
4K Followers 2K Following Open Science Software Enthusiast - Director of Scientific Computing Software at HHMI Janelia - also on Mastodon @[email protected]
Nancy Pelosi Stock Tr.. @PelosiTracker_
561K Followers 224 Following Highlighting Politicians' trades so we can invest alongside Goal: get them banned from trading Powered by @joinautopilot_
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149K Followers 49K Following Science integrity consultant, PhD. #ImageForensics Previously @ Stanford and (gasp!) uBiome. She/her. elisabethbik.bsky https://t.co/V4MR7WVHiT 🇳🇱🇺🇸
Richard McElreath �.. @rlmcelreath
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AI at Meta @metaai
2K Followers 0 Following We've moved, follow us at @AIatMeta for the latest on AI research and innovation from Meta.
Ankit Goyal @imankitgoyal
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Zebrafish Rock! @ZebrafishRock
12K Followers 2K Following Award-winning home tank for all things zebrafish.
GitHub @github
2.6M Followers 341 Following The AI-powered developer platform to build, scale, and deliver secure software.
Jon Daniels @jsdaniel02
945 Followers 454 Following Scientific instrument developer, focused on #lightsheet and other #microscopy at @ASIImaging. 🔬 @[email protected]
Nicole Aponte Santiag.. @NicoleApontePhD
394 Followers 416 Following I am passionate about neurodevelopment, science education, and an advocate for diversity| Post Doc @danwagnerlab @UCSF | Views are my own | She/her 🇵🇷
Kaspar Podgorski @PodgorskiLab
2K Followers 382 Following computational microscopy and tools for brain imaging. Recently moved to @AllenInstitute from @HHMIJanelia
Light-Sheet Fluoresce.. @LSFMMBL
767 Followers 226 Following The Light-Sheet Florescence Microscopy Conference at the Marine Biological Laboratory (@mblscience) in Woods Hole, MA. https://t.co/GIcKHPrFr0
Kyle Harrington @kisharrington
2K Followers 4K Following Images, AI, ML, and ALife. Former: @hampshirecolg @BrandeisU @harvardmed @uidaho @HHMIJanelia @MDC_Berlin @ORNL. Now @ChanZuckerberg. Opinions are mine.
Development @Dev_journal
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Llama 3 70B beats Mistral Large. What exactly Mistral is now supposed to sell?
Our first preprint as a lab is out! This one is short and sweet: We thought there had to be a faster/higher throughput way of making transgenic fish, so we set out to test if lipofection works, and it does!
Lipid-Based Transfection of ZebrafishEmbryos: A Robust Protocol for NucleicAcid Delivery in Zebrafish biorxiv.org/cgi/content/sh… #bioRxiv
Swag! A handheld oscilloscope, almost as nice to have as a shear plate!
While fixing #multiphoton #Microscopes in the lab or building a #mesoSPIM #lightsheet instrument in the field, I often find myself in need of a simple oscilloscope - there are now low-cost multimeter-sized 2 channel oscilloscopes like this Zoyi ZT-703S - superuseful!
@bingy_C : "Great, very easy to use." Okay, let's give it to the PI....
With an incredible opening event with traditional welcoming Okinawan dances and a collaborative opening of awamori the Okinawa Microscopy Workshop has begun! Thrilled to meet so many new colleagues from Japan + SE Asia! So excited to be on the scopes together soon! #OKAscope
Attention organelle enthusiasts!! I am beyond thrilled to introduce Nellie: a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D/3D live-cell microscopy. paper: arxiv.org/abs/2403.13214 github: github.com/aelefebv/nellie 🧵1/N
Some more fun vids (ER, microtubules, and peroxisomes) on @amsikking and @AndrewGYork's SOLS microscope. But Nellie will work on pretty much any data! (Still early, so if it's not working on yours.. lmk, and it will!) (Thanks @AlisterBurt et. al. for the animation plugin) 🧵5/N
Spontaneously blinking fluorophores are great for super-resolution microscopy but a there is no one-size-fits-all dye. Duty-cycle needs vary depending on attachment strategy, labeling density, and technique. So we made a series of eight dyes. biorxiv.org/content/10.110…
Sora is pretty wild. OpenAI team just dropped some new videos 100% AI (minus sound) 1. F1 Race through San Francisco
7 organelles imaged simultaneously in live cancer cells. The complexity of the cell never ceases to amaze me when acquiring these datasets..
I agree. This changes everything. Forget RAG, forget fine-tuning, in-context zero-shot is the way to go. In one year, we will have models with hundreds of millions of tokens of context, if not billions. The problem now is compute. We need new & faster silicon to run these…
Gemini 1.5 pro is STILL under hyped I uploaded an entire codebase directly from github, AND all of the issues (@vercel ai sdk,) Not only was it able to understand the entire codebase, it identified the most urgent issue, and IMPLEMENTED a fix. This changes everything
Gemini 1.5 pro is STILL under hyped I uploaded an entire codebase directly from github, AND all of the issues (@vercel ai sdk,) Not only was it able to understand the entire codebase, it identified the most urgent issue, and IMPLEMENTED a fix. This changes everything
Nate did a really good job with this; it's a solid package that solves an interesting problem. github.com/AndrewGYork/to…
@zacsimile @bingy_C @Daetwyler_St @kD3AN Also a shoutout to @AndrewGYork and Nathaniel H. Thayer from Calico for their open-source concurrency toolkit.
Accelerate real-time cell morphological analysis - pushing everything everywhere all at once! 💨👇🏻 *Real-time cell image acquisition + segmentation @10GB/s using FPGA (see top left) *Real-time (subsampled) DL image classification (green vs yellow cell states) on RTX 4090 GPU…
Absolutely thrilled to continue this exciting, interdisciplinary project with a (now extended) group of amazing scientists: @claire_deo @sylvaingigan @deAguiar_ENS @stiel_lab! Thanks @cziscience for the continued support, looking forward to image deeper @embl using light & sound!
Congratulations to EMBL scientists Claire Deo and Robert Prevedel on receiving a @cziscience Deep Tissue Imaging Phase 2 grant!
Some neat ideas for spot detection from our group, to actually make it work robustly even in challenging imaging conditions ... And everything open-sourced, nicely packaged and ready to spot the spots in your images! Congrats @Albertdm99 and co-authors 👏
Excited to share Spotiflow, our new spot detection method for image-based spatial transcriptomics that facilitates the analysis of large iST images. Lead by @Albertdm99 jointly w @GioeleLaManno @epflSV @EPFL_Imaging @EPFL_BioE biorxiv.org/content/10.110… github.com/weigertlab/spo…
gpt-4 had a slow start on its new year's resolutions but should now be much less lazy now!
Congrats Kevin and team! the #zebrahub ‘metro-map’ produced by #StaVia cartography is absolutely stunning. We have been dreaming of a tool that would fully leverage all aspects of our data to produce rich insightful visualizations and you have done it!
Here’s our new tool #StaVia! It analyzes large single cell atlases (>million) by integrating spatial+temporal info+RNA velocity & scRNAseq. Among atlases, a highlight is the complete #zebrahub metro-map (10hpf->10dpf). Stay tuned for more over weekend 😉 biorxiv.org/content/10.110…
Beautiful work! Congrats @kevin_tsia and the team. So great to see #zebrahub transcriptomic dataset being used so well. #StaVia
Here’s our new tool #StaVia! It analyzes large single cell atlases (>million) by integrating spatial+temporal info+RNA velocity & scRNAseq. Among atlases, a highlight is the complete #zebrahub metro-map (10hpf->10dpf). Stay tuned for more over weekend 😉 biorxiv.org/content/10.110…
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