Howard Salis @hsalis
Assoc Professor of synthetic biology, biological, & chemical engineering. De Novo DNA founder. Engineering microbes with predictive models & algorithms. salislab.net Penn State University Joined April 2009-
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@wc_ratcliff First the scientists developed robots to write papers, and then those robots developed even more robots to write papers. Pretty soon, the AI super intelligent god came to a conclusion: there are too many damned papers and no one is reading them (except me).
It's been 10+ years and there's still not a great multi-proc key-val DB for Python <without running a whole client-server setup>. Dude, I just want to share key-vals across cores (1-100MB) without deadlock.
It's a end-of-semester day of grading & in desperate need of intellectual juicing. Who's got a hot take on gene regulation and would like to start a debate? :)
Congratulations Pamela Silver of @harvardmed, newly inducted #NASmember! #NAS161 #biochemistry #biology
Some exciting news about my team @NatureComms , we're growing and are looking for a synthetic biologist/metabolic engineer: nature.com/naturecareers/…
Markov modeling to show that dynamics of RNA folding & structural ensembles can contribute to stochastic variability in gene expression levels: pubs.aip.org/aip/jcp/articl… Cool.
It is true
How to pick a problem. Today in @CellCellPress. 1/53 cell.com/cell/fulltext/…
Q: has anyone run Qubit on DNA where zeocin antibiotic was used for selection? Both Qubit and zeocin bind to DNA. Is it possible that zeocin could be preventing Qubit from binding? We are seeing very low Qubit conc vs. visual bands on gel and high nanodrop conc.
If you want to be part of this new project to take this work further to make even better microbial leather and other materials - we have several postdoc positions open in the group right now, and more coming soon at Northumbria Uni imperial.ac.uk/jobs/search-jo…
An excellent paper! Authors precisely measure the many factors that affect translation elongation & pausing rates. Many conclusions, my fav: Codon optimality in human cells had little effect, but polyA and poly-lysine paused ribosomes greatly.
An excellent paper! Authors precisely measure the many factors that affect translation elongation & pausing rates. Many conclusions, my fav: Codon optimality in human cells had little effect, but polyA and poly-lysine paused ribosomes greatly.
A recent preprint from our lab about MOSAIC: a very easy protocol to make (short) mutations or libraries in plasmids much easier (no PCR, or assembly cloning needed), just electroporation with oligos! Probably useful for many in the field biorxiv.org/content/10.110… spread the word!
ORCA: Predicting replication origins in circular prokaryotic chromosomes biorxiv.org/cgi/content/sh… #bioRxiv
What does diffusion look like in the crowded bacterial cytoplasm? Is it as weird as people say? Check out our new preprint on 3D tracking of GEMs in live E. coli from @dsvalverdem and @AlpSunol! A fantastic experiment-theory collaboration with @TheZiaLab. biorxiv.org/content/10.110…
Preprint alert! Ever wondered how to precisely program riboswitches? Answer is here @biorxivpreprint:"RNA folding kinetics control riboswitch sensitivity in vivo" Principles for controlling RNA function through controlling RNA folding dynamics. biorxiv.org/content/10.110…
Open Q: what are your fav papers measuring the function of engineered organisms over long time periods (weeks to months)?
Can anyone suggest a list of common cross-linker chemistry used in med chem (for proteins)?
Nice work! Bacteria stack antibiotic resistance markers into many-CDS operons via recombination. Expression levels are determined by changes in translation init rate & mRNA stability (coupled). Good system to study how ribosome protection alters RNase activity in long operons.
Nice work! Bacteria stack antibiotic resistance markers into many-CDS operons via recombination. Expression levels are determined by changes in translation init rate & mRNA stability (coupled). Good system to study how ribosome protection alters RNase activity in long operons.
Tom Ellis @ProfTomEllis
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13K Followers 2K Following Join @SynBioBeta - May 6-9, 2024 in San Jose: https://t.co/s9oQOoa6Cg and read my weekly newsletter: https://t.co/wlPcmG0KfGKatie Galloway @GallowayLabMIT
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17 Followers 253 Following BSc | MSc Biochem | interest-Bioinformatics---Computational Biology.Double Wu @XiaoPPh
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120 Followers 213 Following PhD Candidate in the Department of Biochemistry and Molecular Biology at Michigan State University. Research centers on the metabolic modeling of plants. He/HimTom Ellis @ProfTomEllis
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7K Followers 483 Following Professor of Bioengineering, Stanford UniversityNiko McCarty 🧫 @NikoMcCarty
23K Followers 861 Following Writing and editing stories about scientific progress, especially in biology. Founding Editor @AsimovPress Head of Creative @AsimovBioTae Seok Moon @Moon_Synth_Bio
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1K Followers 3K Following Postdoc @MRC_TU @Cambridge_Uni 🇬🇧 PhD @SMS_UWA 🇦🇺 Biotech @UNQoficial 🇦🇷 #Microbiology #EngineeringBiology #ChemicalGenetics 🧬She/her Latinx@breakliquid @moravec_tomas each time you freeze thaw the T4 ligase buffer the ATP degrades some more. I make 10mM ATP stocks and just keep small aliquots. Cutsmart + ATP seems to work better than T4 Ligase Buffer (which has ATP)
I’m a scholar of the Israeli-Palestinian conflict and a longtime advocate for peace and for Palestinian rights. I have publicly opposed Israel’s occupation of the West Bank, and its blockade and now destruction of Gaza. I support the rights of students and faculty to peacefully…
A multiplexed and quantitative assay for small-molecule detection via photo-crosslinking of structure switching aptamers biorxiv.org/cgi/content/sh… #bioRxiv
@hsalis Reiter and Stark recently took some enhancers and just swapped the TFBSs with other TFBSs and they drive similar activity (in S2 cells at least...), so it seems more like enhancers simply overcome an activation "energy barrier," so to speak. No need for grammar...
@hsalis Okay I'll bite. Apart from a few exceptions (I.e. Cooperative TF pairs), "enhancer grammar," probably doesn't exist.
Congratulations Pamela Silver of @harvardmed, newly inducted #NASmember! #NAS161 #biochemistry #biology
Them: so how's it going? Me: we're really on a roll The roll 👇 🤣 (sound on)
Some exciting news about my team @NatureComms , we're growing and are looking for a synthetic biologist/metabolic engineer: nature.com/naturecareers/…
Unveiling the molecular dance: how a small protein tames the transcription terminator Rho communities.springernature.com/posts/unveilin…
@hsalis @GenScript To be clear, I’m proposing that people show up at the requestor’s facility and use requestor stuff in their spare time.
@hsalis @vivek_mutalik I appreciate your suggestion for perturbing rho activity or rut sites in the gene! Also, we would be very interested in measuring % of prematurely released transcripts in your constructs. Your paper shows many interesting constructs for this test. /n
@hsalis @sjkimlab @vivek_mutalik Long story short, it's about time other disciplines beyond CH E learned to take chemical kinetics seriously.
@hsalis @OmicsOmicsBlog Try our Tech Support team: [email protected] R&D/Product Manager may be able to confirm this for you.
@hsalis @OmicsOmicsBlog The specific binding of DNA to the dye in the Qubit kit makes it more reliable than nanodrop. Perhaps the experts at the producing company may be aware of the possibility of other components binding DNA and competing the dye!!
How to pick a problem. Today in @CellCellPress. 1/53 cell.com/cell/fulltext/…