Brett Chrest @Chrest_brett
PhD student with expertise in Mitochondrial Bioenergetics & Cancer Metabolism with @KFW_Lab┃ Focus: mSLP, Respiratory & Energy Limitations in AML🩸 Winston Salem, NC Joined July 2013-
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Slight caution: Labeling glutamine, blocking glutaminase, and *only* looking at labeled extracellular succinate might not give clear insight into the mitochondrial pathway. AKG can be exported & converted to succinate outside of the mito. nature.com/articles/s4146…
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Successful day, love getting results that make me question the traditional 'cycle' of the TCA cycle 🤓
Brett Chrest retweeted
And here how brain tumors exploit glucose for their growth, along with a dietary restriction of an amino acid therapy to prevent it nature.com/articles/s4158…
Caution around isolated enzyme experiments, I love this example: SUCL activity is due to succinyl-CoA production from AKGDH. Thus you can genetically down regulate AKGDH, isolate mitochondria and test maximal SUCL activity and see no difference. pmc.ncbi.nlm.nih.gov/articles/PMC36…
Brett Chrest retweeted
We present our MetaProViz #Rpackage for #metabolomics analysis & prior knowledge integration to generate mechanistic hypotheses on how metabolic changes affect metabolite classes, pathways & environment interaction 🔗biorxiv.org/content/10.110… 📦 saezlab.github.io/MetaProViz/ 🧵 Thread
Brett Chrest retweeted
Discovery happens less when you're trying to be the expert and more when you're trying to be the learner.
Brett Chrest retweeted
Congratulations to @mlvalenstein and Max Wranik and senior author Kacper Rogala on this beautiful work showing in atomic detail how amino acids activate mTORC1 via GATOR2. nature.com/articles/s4158…
Brett Chrest retweeted
A great thing about working in a lab is being involved in lab meetings and witnessing everyone's projects take shape. A single project may seem to go slow but all projects together is impressive. Caroline Bartman discussed this on the podcast: podcasts.apple.com/us/podcast/28-…
Common thought is diabetes & obesity would be worse for cancer due to increased supply of glucose for the tumor. However, using a breast tumor model, diabetic obese mice have no difference in tumor glucose metabolism compared to control diet. link.springer.com/article/10.100…
Brett Chrest retweeted
The goal of a PhD is not to learn some facts or read a few papers or learn a bunch of techniques. The goal of a PhD is to learn independence, problem solving, how to finish things you start, resilience, & gain the ability to adapt & think creatively. Learning these things is hard
Brett Chrest retweeted
@slavov_n While whole-genome sequencing using Illumina is commonly described as such, in reality, it is closer to half genome sequencing due to limitations of sequencing (GC content) coverage, not to mention variant calling limitations that you allude to.
Brett Chrest retweeted
more negative metformin and cancer data. when will this end?
more negative metformin and cancer data. when will this end?
Brett Chrest retweeted
MOSAIC large field of view 3D single molecule localization microscopy of mitochondrial outer membranes and nuclear membranes in fixed U2OS cells.
Brett Chrest retweeted
According to DNA methylation "aging clocks", physical activity "rejuvenates" athletes immediately after the game. 𝐔𝐩𝐨𝐧 𝐫𝐞𝐬𝐭, 𝐭𝐡𝐞 𝐫𝐞𝐣𝐮𝐯𝐞𝐧𝐚𝐭𝐢𝐨𝐧 𝐝𝐢𝐬𝐚𝐩𝐩𝐞𝐚𝐫𝐬. This is inconsistent with my definition of aging. 🧵
I remember when this came out and never read it fully. Despite the title, BHB did not change overall mitochondrial function. CII is restored in critically-ill based conditions, but hard to interpret with saturating succinate + succinate from ketolysis sciencedirect.com/science/articl…
Brett Chrest retweeted
Researchers led by Ludwig @Princeton's @lynchielydia have discovered that in obese people, who are at high risk for many cancers, a diet rich in animal fats—but not plant-derived fats—suppresses antitumor immunity and promotes cancer progression. bit.ly/4fcfWu9
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Katherine Ansley, MD @KatherineAnsley
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